Imaging Core

Chris Buser

Senior Faculty – Electron & Atomic Force Microscopy

Dipl. Natw. ETH (MS); Biochemistry; Swiss Federal Institute of Technology Zurich; 2003

Ph.D.; Human Biology; University of Ulm, Germany; 2006

Postdoctoral Scholar; Molecular & Cell Biology; UC Berkeley; 2007-2011

Principal Research Interests

  • High-resolution imaging in biomedical research
  • Development of microscopy preparation techniques
  • Infectious diseases & diagnostics
  • Startups & ecosystem development

Representative Publications

  • Perry S, Goel P, Tran NL, Pinales C, Buser C, Miller DL, Ganetzky B, Dickman D. Developmental arrest of Drosophila larvae elicits presynaptic depression and enables prolonged studies of neurodegeneration. Development. 2020 May 21;147(10):dev186312. doi: 10.1242/dev.186312. PMID: 32345746.
  • Ding K, Han Y, Seid TW, Buser C, Karigo T, Zhang S, Dickman DK, Anderson DJ. Imaging neuropeptide release at synapses with a genetically engineered reporter. Elife. 2019 Jun 26;8:e46421. doi: 10.7554/eLife.46421. Erratum in: Elife. 2019 Dec 11;8: PMID: 31241464; PMCID: PMC6609332.
  • Buser C and Drubin DG. 2013. Ultrastructural imaging of endocytic sites in S. cerevisiae by transmission electron microscopy and immunolabeling. Microsc Microanal., 19(2):381-92.
  • Kishimoto T, Sun Y, Buser C, Liu J, Michelot A, Drubin DG. 2011. Determinants of endocytic membrane geometry, stability and scission. PNAS108(44):E979-88.
  • McDonald K, Schwarz H, Müller‐Reichert T, Webb R, Buser C, Morphew M. 2010. ‘Tips and tricks’ for high‐pressure freezing of model systems. Methods Cell Biol., 96:671-93.
  • Popa M, Ruzsics Z, Lötzerich M, Dölken L, Buser C, Walther P, Koszinowski UH. 2010. Dominant negative mutants of murine cytomegalovirus M53 gene block nuclear egress and inhibit capsid maturation. J Virol, 84(18):9035-46.
  • Buser C, McDonald K. 2010. Correlative GFP-immunoelectron microscopy in yeast. Methods Enzymol. 470: 603-18.
  • Chevillotte M, Landwehr S, Linta L, Frascaroli G, Lüske A, Buser C, Mertens T, von Einem J. 2008. The major tegument protein pp65 of human cytomegalovirus is required for the incorporation of pUL69 and pUL97 into the virus particle and for viral growth in macrophages. J Virol 83(6):2480-90.
  • Buser C, Walther P. 2008. Freeze-Substitution: The Addition of Water to Polar Solvents Enhances the Retention of Structure and Acts at Temperatures Around –60 °C. J Microsc 230 (Pt 2):268-77.
  • Buser C, Walther P, Mertens T, Michel D. 2007. Cytomegalovirus primary envelopment occurs at large infoldings of the inner nuclear membrane. J Virol 81:3042-8.
  • Rupp B, Ruzsics Z, Buser C, Adler B, Walther P, Koszinowski UH. 2007. A random screen for dominant-negative mutants of the cytomegalovirus nucler egress protein M50. J Virol 81:5508-17.